Composite

Part:BBa_K415151:Design

Designed by: Shirley Galbiati   Group: iGEM10_MIT   (2010-10-24)


K415010 + RBS : p8-GR1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2368
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Linker 18 picked as one of the 15 amino acid linkers to enhance high copy protein display. opti-p8 was chosen for the same reason.


Source

Sources: RBS: BBa_B0034 p8-leader sequence: First 23 amino acids of p8, exact sequenced derived from M13KE. GR1 coil: from Wang C, Zhong P, Wang X. Adapter-directed display systems. In Vitro. 2007;17(12). linker sequence: Linker 18 from Weiss et al. Mutational analysis of the major coat protein of M13 identifies residues that control protein display. 2000 opti-p8:from Weiss et al. 2000, derived from M13KE


The source of the p8-leader sequence is M13KE. The optimized p8 is derived from M13KE3 (see Weiss et al. Mutational analysis of the major coat protein of M13 identifies residues that control protein display. 2000).

References